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c rodentium strain dbs100 (ATCC)

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ATCC c rodentium strain dbs100
C Rodentium Strain Dbs100, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+rodentium+strain+dbs100/Citrobacter+rodentium%3B+Strain+DBS100/pm41723158-324-9-13
Average 96 stars, based on 261 article reviews

c rodentium strain dbs100 - by Bioz Stars, 2026-09

96/100 stars

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Infection:

Article Title: Reduced intestinal GLP-1 + cell numbers are associated with an inflammation-related epithelial metabolic signature.
Article Snippet: .. The C. rodentium strain DBS100 (ATCC 51459; American Type 538 Culture Collection) was used for infection as previously described (104). ..

Article Title: RORγt + APCs require a distinct cis-regulatory element to instruct tolerance to dietary antigens.
Article Snippet: .. Citrobacter rodentium infection model Mice were orally gavaged with C. rodentium strain DBS100 (ATCC 51459). ..

Article Title: Reduced intestinal GLP-1 + cell numbers are associated with an inflammation-related epithelial metabolic signature
Article Snippet: .. The C. rodentium strain DBS100 (ATCC 51459; American Type Culture Collection) was used for infection as previously described ( ). ..

Article Title: Reduced Intestinal GLP-1 + Cell Numbers Are Associated With an Inflammation-related Epithelial Metabolic Signature
Article Snippet: .. The C rodentium strain DBS100 (ATCC 51459; American Type Culture Collection) was used for infection as previously described. ..

Mouse Assay:

Cell Culture:

Article Title: The IL-21/IL-21R signaling axis regulates CD4+ T-cell responsiveness to IL-12 to promote bacterial-induced colitis.
Article Snippet: The stimulated cells were harvested after 1 h and 4 h by centrifugation at 900 × g for 5 min and washed twice with ice-cold 1× phosphate-buffered saline (PBS), followed by the total RNA extraction using the RNeasy Micro Kit (Qiagen) for Nanostring analysis. .. C. rodentium strain DBS100 (ATCC 51459) was obtained from the American Type Culture Collection and cultured in Luria–Bertani (LB; Sigma-Aldrich) broth at 37 °C overnight with shaking at 200 rpm. .. Small aliquots (200 μL) of the overnight bacterial cultures were transferred into 50 mL Falcon® tubes (BD Falcon) containing fresh LB medium and were further propagated at 37 °C with shaking at 200 rpm.

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a – c , Immunoblots of Caco-2 cells infected by E. coli strain EDL933. Infection with WT or NleL deficient strains ( a ). Where indicated, cells were cultured in 1 µM bortezomib (Btz) ( b ) or 10 µg ml −1 TRAIL ( c ). hpi, hours post-infection. Results are representative of three independent experiments. MOI, multiplicity of infection; p, phosphorylated; c, cleaved. d , Scheme for quantifying the cellular extrusion of dead PI + IECs from monolayers. e – g , m , Nearest neighbour internuclear distances in IEC monolayers. e , Cells from WT IEC organoids were treated for 30 min with FlaTox and DMSO vehicle, 2.5 mM cytochalasin D (CytoD) or 10 µM Y-27632. n = 8 monolayers per group. f , Cells from Rock1- and Rock2- knockout IEC organoids were stimulated with FlaTox. n = 4 monolayers per group. g , m , Cells from WT ( g ) or Villin.CreERT2 control and Rock1- and Rock2- knockout ( m ) IEC organoids were infected with Citrobacter strain DBS100 (WT, Δ nleL or +NleL). n = 4 monolayers per group. P values determined by two-tailed Mann–Whitney test. NS, not significant. Results are representative of three independent experiments. h , Quantification of extrusion time of individual cells in live-imaged WT IEC monolayers infected with Citrobacter strain DBS100 (Δ nleL or +NleL). P values determined by two-tailed Mann–Whitney test. Results are representative of two independent experiments. i – k , Colony-forming units (CFUs) in the mouse colon ( i ), colon lumen ( j ) and spleen ( k ) six days after infection with Citrobacter strain DBS100. Circles, different mice (data pooled from two experiments, n = 10 mice per group); lines, mean. P values determined by two-way ANOVA. LOD, limit of detection. l , Immunoblots of colon IECs from mice infected by Citrobacter strain DBS100. Results are representative of three independent experiments. n , Representative confocal z -stacks of the IEC cultures in m . Scale bar, 30 μm. For gel source data, see Supplementary Fig. .

Journal: Nature

Article Title: Enteropathogenic bacteria evade ROCK-driven epithelial cell extrusion

doi: 10.1038/s41586-025-09645-0

Figure Lengend Snippet: a – c , Immunoblots of Caco-2 cells infected by E. coli strain EDL933. Infection with WT or NleL deficient strains ( a ). Where indicated, cells were cultured in 1 µM bortezomib (Btz) ( b ) or 10 µg ml −1 TRAIL ( c ). hpi, hours post-infection. Results are representative of three independent experiments. MOI, multiplicity of infection; p, phosphorylated; c, cleaved. d , Scheme for quantifying the cellular extrusion of dead PI + IECs from monolayers. e – g , m , Nearest neighbour internuclear distances in IEC monolayers. e , Cells from WT IEC organoids were treated for 30 min with FlaTox and DMSO vehicle, 2.5 mM cytochalasin D (CytoD) or 10 µM Y-27632. n = 8 monolayers per group. f , Cells from Rock1- and Rock2- knockout IEC organoids were stimulated with FlaTox. n = 4 monolayers per group. g , m , Cells from WT ( g ) or Villin.CreERT2 control and Rock1- and Rock2- knockout ( m ) IEC organoids were infected with Citrobacter strain DBS100 (WT, Δ nleL or +NleL). n = 4 monolayers per group. P values determined by two-tailed Mann–Whitney test. NS, not significant. Results are representative of three independent experiments. h , Quantification of extrusion time of individual cells in live-imaged WT IEC monolayers infected with Citrobacter strain DBS100 (Δ nleL or +NleL). P values determined by two-tailed Mann–Whitney test. Results are representative of two independent experiments. i – k , Colony-forming units (CFUs) in the mouse colon ( i ), colon lumen ( j ) and spleen ( k ) six days after infection with Citrobacter strain DBS100. Circles, different mice (data pooled from two experiments, n = 10 mice per group); lines, mean. P values determined by two-way ANOVA. LOD, limit of detection. l , Immunoblots of colon IECs from mice infected by Citrobacter strain DBS100. Results are representative of three independent experiments. n , Representative confocal z -stacks of the IEC cultures in m . Scale bar, 30 μm. For gel source data, see Supplementary Fig. .

Article Snippet: EHEC O157:H7 EDL933 (ATCC700927) and C. rodentium DBS100 (ATCC51459) were obtained from the American Type Culture Collection (ATCC).

Techniques: Western Blot, Infection, Cell Culture, Knock-Out, Control, Two Tailed Test, MANN-WHITNEY

a , Immunoblots of 293T cells transfected with human or mouse ROCK1/2 and NleL. Results representative of 3 independent experiments. b , Number of GFP-expressing Citrobacter bacteria adhered to WT IEC monolayers. Data are mean ± s.d. Circles, a single image field (n = 11 per condition taken from n = 4 monolayers per group). P values determined by one-way ANOVA. c , Average distance of GFP+ bacteria from nearest PI+ nucleus, compared to epithelial cell diameter, related to transwell monolayer data in Fig. . n = 12 monolayers with representative images demonstrating bacterial infection of PI+ cells. Scale bar is 20 µm. d , Quantification of % PI+ cells among all cells in uninfected versus Citrobacter strain DBS100 WT infected monolayers. Circles, single image field (no infection, n = 8 and Citrobacter n = 9) from 3 separate monolayers. P values determined by two-tailed Mann–Whitney test. Results representative of 3 independent experiments. e , Extrusion imaging of individual cells in live-imaged monolayers infected with Citrobacter strain DBS100 (Δ nleL or + nleL ). Representative frames from imaging data used for quantification in Fig. . Blue circles mark extruding cell analysed from start to finish. Scale bar = 5 µm. Results representative of 2 independent experiments. f , Immunoblot of IECs collected from individual mice in (g). g , FITC–dextran concentration in plasma and faeces. Circles, individual mice (n = 5 mice per group). Data are mean ± s.d. of 3 independent experiments. h – j , CFUs in the colons ( h , i ), and caecum lumens ( j ) of mice infected with Citrobacter strain DBS100 WT or Δ nleL for 10 ( h ) or 6 days ( i , j ). Circles, individual mice ( h , i n = 5 mice per group, j , data pooled from 2 experiments, n = 10 mice per group, LOD=limit of detection). Lines indicate the mean. P values determined by two-way ANOVA. k , l , Secreted IL-18 ( k ) or cell death ( l ) from IECs infected by Citrobacter Δ nleL or +NleL in Fig. . Circles, individual transwells of IEC organoids ( k , n = 11 and l , n = 8). Data are mean ± s.d. P values determined by two-tailed Mann–Whitney test. Results representative of 3 independent experiments. m , Transepithelial electrical resistance in transwells (circles, n = 20) of IEC organoids. Box plot shows the median, interquartile range and minimum to maximum values (whiskers). P values determined by two-tailed Mann–Whitney test. Results representative of 3 independent experiments. For gel source data, see Supplementary Fig. .

Journal: Nature

Article Title: Enteropathogenic bacteria evade ROCK-driven epithelial cell extrusion

doi: 10.1038/s41586-025-09645-0

Figure Lengend Snippet: a , Immunoblots of 293T cells transfected with human or mouse ROCK1/2 and NleL. Results representative of 3 independent experiments. b , Number of GFP-expressing Citrobacter bacteria adhered to WT IEC monolayers. Data are mean ± s.d. Circles, a single image field (n = 11 per condition taken from n = 4 monolayers per group). P values determined by one-way ANOVA. c , Average distance of GFP+ bacteria from nearest PI+ nucleus, compared to epithelial cell diameter, related to transwell monolayer data in Fig. . n = 12 monolayers with representative images demonstrating bacterial infection of PI+ cells. Scale bar is 20 µm. d , Quantification of % PI+ cells among all cells in uninfected versus Citrobacter strain DBS100 WT infected monolayers. Circles, single image field (no infection, n = 8 and Citrobacter n = 9) from 3 separate monolayers. P values determined by two-tailed Mann–Whitney test. Results representative of 3 independent experiments. e , Extrusion imaging of individual cells in live-imaged monolayers infected with Citrobacter strain DBS100 (Δ nleL or + nleL ). Representative frames from imaging data used for quantification in Fig. . Blue circles mark extruding cell analysed from start to finish. Scale bar = 5 µm. Results representative of 2 independent experiments. f , Immunoblot of IECs collected from individual mice in (g). g , FITC–dextran concentration in plasma and faeces. Circles, individual mice (n = 5 mice per group). Data are mean ± s.d. of 3 independent experiments. h – j , CFUs in the colons ( h , i ), and caecum lumens ( j ) of mice infected with Citrobacter strain DBS100 WT or Δ nleL for 10 ( h ) or 6 days ( i , j ). Circles, individual mice ( h , i n = 5 mice per group, j , data pooled from 2 experiments, n = 10 mice per group, LOD=limit of detection). Lines indicate the mean. P values determined by two-way ANOVA. k , l , Secreted IL-18 ( k ) or cell death ( l ) from IECs infected by Citrobacter Δ nleL or +NleL in Fig. . Circles, individual transwells of IEC organoids ( k , n = 11 and l , n = 8). Data are mean ± s.d. P values determined by two-tailed Mann–Whitney test. Results representative of 3 independent experiments. m , Transepithelial electrical resistance in transwells (circles, n = 20) of IEC organoids. Box plot shows the median, interquartile range and minimum to maximum values (whiskers). P values determined by two-tailed Mann–Whitney test. Results representative of 3 independent experiments. For gel source data, see Supplementary Fig. .

Article Snippet: EHEC O157:H7 EDL933 (ATCC700927) and C. rodentium DBS100 (ATCC51459) were obtained from the American Type Culture Collection (ATCC).

Techniques: Western Blot, Transfection, Expressing, Bacteria, Infection, Two Tailed Test, MANN-WHITNEY, Imaging, Concentration Assay, Clinical Proteomics

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